dmirb fluorescence microscope (Leitz GmbH)
90
Structured Review
Leitz GmbH
dmirb fluorescence microscope
Dmirb Fluorescence Microscope, supplied by Leitz GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dmirbe+microscope/dmirb+fluorescence+microscope/pm40421877-60-15-15
Average 90 stars, based on 1 article reviews
Dmirb Fluorescence Microscope, supplied by Leitz GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dmirbe+microscope/dmirb+fluorescence+microscope/pm40421877-60-15-15
Average 90 stars, based on 1 article reviews
dmirb fluorescence microscope - by Bioz Stars,
2026-11
90/100 stars
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Staining:Article Title: The effects of red LED light on pig sperm function rely upon mitochondrial electron chain activity rather than on a PKC-mediated mechanism Article Snippet: .. Not only were sperm stained with JC-1 analyzed through flow cytometry, but they were also observed under a Leica TCS 4D confocal laser scanning microscope (Leica Lasertechnik; Wertrieb, Germany) adapted to an inverted Article Title: 'In vitro' capacitation and acrosome reaction are concomitant with specific changes in mitochondrial activity in boar sperm: evidence for a nucleated mitochondrial activation and for the existence of a capacitation-sensitive subpopulational structure. Article Snippet: A 1.5-ml aliquot of sperm was incubated with 15 ll of JC-1 staining (153 lM; T-3168; Molecular Probes, Eugene, OR, USA) for 10 min in the dark at 37 C. The sperm was placed on clean microscope slides with a coverslip on the top. .. Images of the live stained 666 L Ramió-Lluch, JM Fernández-Novell, A Peña, C Colás, JA Cebrián-Pérez, T Muiño-Blanco, A Ramı́rez, II Concha, T Rigau and JE Rodrı́guez-Gil 2010 Blackwell Verlag GmbH sperm were obtained with a Leica TCS 4D confocal scanning microscope (Leica Lasertechnik GmbH; Heidelberg, Germany) adapted to an inverted Article Title: The thiol oxidant dipyridyl disulfide can supply the PDI-Ero1p pathway with additional oxidative equivalents. Article Snippet: Membrane-permeant oxidants have become a standard tool for studying eukaryotic organisms because they affect the redox state and the redox regulation of different compartments.. The ero1-1 mutant is temperature sensitive (378C) and cannot grow under anaerobic conditions.. Low micromolar concentrations of the specific thiol-oxidant dipyridyl disulfide (DPS) rescue both these growth defects. Flow Cytometry:Article Title: The effects of red LED light on pig sperm function rely upon mitochondrial electron chain activity rather than on a PKC-mediated mechanism Article Snippet: .. Not only were sperm stained with JC-1 analyzed through flow cytometry, but they were also observed under a Leica TCS 4D confocal laser scanning microscope (Leica Lasertechnik; Wertrieb, Germany) adapted to an inverted Laser-Scanning Microscopy:Article Title: The effects of red LED light on pig sperm function rely upon mitochondrial electron chain activity rather than on a PKC-mediated mechanism Article Snippet: .. Not only were sperm stained with JC-1 analyzed through flow cytometry, but they were also observed under a Leica TCS 4D confocal laser scanning microscope (Leica Lasertechnik; Wertrieb, Germany) adapted to an inverted Article Title: Mfn2 downregulation in excitotoxicity causes mitochondrial dysfunction and delayed neuronal death Article Snippet: .. Imaging studies Cells were visualized using a TCS SP2 Leica confocal laser scanning microscope (Leica Lasertechnick GmbH, Mannheim, Germany) adapted to an inverted Microscopy:Article Title: The effects of red LED light on pig sperm function rely upon mitochondrial electron chain activity rather than on a PKC-mediated mechanism Article Snippet: .. Not only were sperm stained with JC-1 analyzed through flow cytometry, but they were also observed under a Leica TCS 4D confocal laser scanning microscope (Leica Lasertechnik; Wertrieb, Germany) adapted to an inverted Article Title: Agonist-induced internalization of mGluR1alpha is mediated by caveolin. Article Snippet: Tissues were labeled for 16 h at 4 C with mouse anti-mGluR1a (BD Bioscience) and rabbit anti-caveolin (BD Bioscience) antibodies in blocking solutions, washed, and incubated with goat anti-mouse Alexa 488 (Molecular Probes, Eugene, OR, USA) and goat anti-rabbit Alexa 594 (Molecular Probes) secondary antibodies for 1 h at 22–25 C. The tissues were washed and mounted with Gel/Mount (Biomeda, Burlingame, CA, USA). .. Confocal microscope observations were made with a LeicaTCSSP (Leica Lasertechnik GmH, Heidelberg, Germany) confocal scanning laser microscope adapted to an inverted Article Title: "In vitro" capacitation and subsequent acrosome reaction are related to changes in the expression and location of midpiece actin and mitofusin-2 in boar spermatozoa. Article Snippet: The induction of “in vitro” capacitation (IVC) and subsequent, progesterone-induced “in vitro” acrosome reaction (IVAR) was concomitant with an increase in actin polymerization, also showing an increase in actin presence at the apical area of the midpiece.. The presence of mitofusin-2, a protein involved in the regulation of the coordinated mitochondrial function, expanded from midpiece to the principal piece after IVC and IVAR.. All of these results indicate that the increase of boar sperm mitochondrial activity during IVC and the first minutes of IVAR is concomitant with changes in the expression and location of both actin and mitofusin-2. Article Title: 'In vitro' capacitation and acrosome reaction are concomitant with specific changes in mitochondrial activity in boar sperm: evidence for a nucleated mitochondrial activation and for the existence of a capacitation-sensitive subpopulational structure. Article Snippet: A 1.5-ml aliquot of sperm was incubated with 15 ll of JC-1 staining (153 lM; T-3168; Molecular Probes, Eugene, OR, USA) for 10 min in the dark at 37 C. The sperm was placed on clean microscope slides with a coverslip on the top. .. Images of the live stained 666 L Ramió-Lluch, JM Fernández-Novell, A Peña, C Colás, JA Cebrián-Pérez, T Muiño-Blanco, A Ramı́rez, II Concha, T Rigau and JE Rodrı́guez-Gil 2010 Blackwell Verlag GmbH sperm were obtained with a Leica TCS 4D confocal scanning microscope (Leica Lasertechnik GmbH; Heidelberg, Germany) adapted to an inverted Article Title: Mfn2 downregulation in excitotoxicity causes mitochondrial dysfunction and delayed neuronal death Article Snippet: .. Imaging studies Cells were visualized using a TCS SP2 Leica confocal laser scanning microscope (Leica Lasertechnick GmbH, Mannheim, Germany) adapted to an inverted Article Title: The thiol oxidant dipyridyl disulfide can supply the PDI-Ero1p pathway with additional oxidative equivalents. Article Snippet: Membrane-permeant oxidants have become a standard tool for studying eukaryotic organisms because they affect the redox state and the redox regulation of different compartments.. The ero1-1 mutant is temperature sensitive (378C) and cannot grow under anaerobic conditions.. Low micromolar concentrations of the specific thiol-oxidant dipyridyl disulfide (DPS) rescue both these growth defects. Article Title: Intracellular calcium movements of boar spermatozoa during 'in vitro' capacitation and subsequent acrosome exocytosis follow a multiple-storage place, extracellular calcium-dependent model. Article Snippet: .. Samples were visualized under a Leica TCS 4D confocal scanning microscope (Leica Lasertechnik; Vertrieb, Germany) adapted to an inverted Article Title: FATP1 localizes to mitochondria and enhances pyruvate dehydrogenase activity in skeletal myotubes. Article Snippet: Fatty acid transport protein 1 (FATP1) has been previously immunolocalized in intracellular compartments.. Here we show that FATP1 localizes to the mitochondria in cultured myotubes, by immunoblots of subcellular fractions and immunocytology of the fusion protein FATP1-GFP.. FATP1 strongly stimulates CO2 production from glucose whereas nonmitochondrial metabolism of glucose is only slightly enhanced. Imaging:Article Title: Mfn2 downregulation in excitotoxicity causes mitochondrial dysfunction and delayed neuronal death Article Snippet: .. Imaging studies Cells were visualized using a TCS SP2 Leica confocal laser scanning microscope (Leica Lasertechnick GmbH, Mannheim, Germany) adapted to an inverted Fluorescence:Article Title: FATP1 localizes to mitochondria and enhances pyruvate dehydrogenase activity in skeletal myotubes. Article Snippet: Fatty acid transport protein 1 (FATP1) has been previously immunolocalized in intracellular compartments.. Here we show that FATP1 localizes to the mitochondria in cultured myotubes, by immunoblots of subcellular fractions and immunocytology of the fusion protein FATP1-GFP.. FATP1 strongly stimulates CO2 production from glucose whereas nonmitochondrial metabolism of glucose is only slightly enhanced. |